1mg/Vial Human Growth Follistatin 344 for Muscle Mass Iincreasing

Model NO.: Follistatin 344 Customized: Non-Customized Suitable for: Elderly, Children, Adult Purity: >99% Synonyms: Fst, Fs, Activin-Binding Protein Single Impurity: 0.08% Packing: 1mg/Vial, 2mg/Vial Specification: 1mg/vial HS Code: 29224999 Powder: Yes Certification: GMP, HSE, ISO 9001, USP, BP State: Solid Name: Follistatin 344 Total Impurities: 0.23% Source: Escherichia Coli. Trademark: Shuangbojie Origin: China 1mg/Vial Human Growth Follistatin 344 for Muscle Mass Iincreasing

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Description	Follistatin Human Recombinant produced in E.Coli is a single, non-glycosylated  polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa. The FST is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a concentrated (1mg/ml) solution containing no additives.
Solubility	It is recommended to reconstitute the lyophilized Follistatin in sterile 18MΩ-cm H2O  not less than 100g/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Follistatin although stable at room temperature for 3 weeks, should be  stored desiccated below -18 ºC. Upon reconstitution FST should be stored at 4ºC  between 2-7 days and for future use below -18ºC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence	The sequence of the first five N-terminal amino acids was determined and was found  to be Gly-Asn-Cys-Trp-Leu.
Biological Activity	The activity is determined by the ability to neutralize Activin A inhibitory effect of mouse  MPC-11 cells. The expected ED50 is 0.1-0.4 g/ml in the presence of 7.5 ng/ml Activin A.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household  chemicals.

Materials and Methods

Animals.

C57BL/6, C57BL/10, and C57BL/10ScSn-DMDmdx/J were purchased from The Jackson Laboratory. All studies were approved by Institutional Animal Care and Use Committee.

Cloning and AAV Production.

The cDNA for human follistatin-344 (FS) was obtained from Origene, follistatin-related gene (FLRG) was obtained from American Type Culture Collection, and growth and differentiation factor-associated serum protein 1 (GASP-1) was cloned from a human cDNA library (Clontech). Recombinant AAV serotype 1 vectors were produced by a contract manufacturing company (Virapur).

AAV Injections and Testing.

Mice received bilateral intramuscular injections of a total dose of 1 × 1011 viral particles (high = 1 × 1011, low = 1 × 1010) (n = 10-15 per group) at 3-4 weeks of age or at 6.5 months of age. Muscle strength was assessed weekly, using a grip strength meter (27). Force measurements were recorded in three separate trials and averaged. Mouse coordination was tested by using the accelerating rotarod (Columbus Instruments).

Histological Analysis.

Muscles were dissected, weighed, snap-frozen in liquid nitrogen-cooled isopentane, cryostat sectioned, and stained by hematoxylin-eosin (H&E) or succinic dehydrogenase (SDH) for analysis of fiber diameters. Five animals per group were chosen randomly for muscle fiber size morphometry. For each analysis, five representative pictures (one central and four peripheral) were taken of muscle sections, compounding to 0.7 mm2. Images were captured at ×20 magnification, and diameters were measured with a calibrated micrometer, using the AxioVision 4.2 software (Zeiss). Fiber size-distribution histograms were generated and expressed as percentage of total fibers analyzed.

Creatine Kinase and Follistatin Assay.

Serum CK was performed by using a CK test kit (Pointe Scientific) and expressed as units/liter. Serum was collected at 90 days after injection and assayed by using the human follistatin quantikine ELISA kit (R&D Systems) with normalization to controls.

Statistical Analysis.

All statistical analysis was performed in Graph Pad Prizm software, using one- and two-way ANOVA with Bonferroni post hoc analysis.

Follistatin 344
Synonyms:FST, FS, Activin-binding protein.
Appearance:Sterile Filtered White Lyophilized (Freeze-Dried)
Purity(by HPLC):>99.00%~99.90%
Total Impurities(by HPLC):1.00%~0.05%
Single Impurity(by HPLC):

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